Five-hour half-life of mouse liver gap-junction protein
نویسندگان
چکیده
The half-life of a gap-junction polypeptide band migrating at 21,000 Mr on SDS polyacrylamide gels isolated from mouse liver is measured to be 5 h. Two low-molecular wight bands, probably related to the 21,000 Mr material by proteolysis, have measured half-lives of 4.6 and 5.2 h. Gap junctions are labeled in vivo using the 14C-bicarbonate labeling procedure, followed by quantitative fluorography.
منابع مشابه
Phosphorylated and non-phosphorylated connexin-32 molecules in gap junction plaques are protected against calpain proteolysis after phosphorylation by protein kinase C.
Introduction Gap junctions are formed by plaques of functionally assembled intercellular channels which are responsible for direct communication between the cytosols of adjacent cells in the form of ions and secondmessenger molecules. These channels are formed as two hexameric hemichannels of connexin (cnx) subunits that interlock head-on [ 1-61, Connexiris form an extensive family of well cons...
متن کاملGene Expression Profile Analysis during Mouse Tooth Development
Introduction: Complex molecular pathways involve in development of different tissues such as teeth. Differential gene expression patterns during teeth development generates different tooth types. Teeth development results from interactions between oral epithelium and underlying ectomesenchyme cells with neural crest origin. Teeth development are regulated by different signaling networks. In thi...
متن کاملUltrastructural characterization of the death process of hepatocytes in neonatal mouse liver
Introduction Hematopoiesis in mouse fetal liver starts at 10 days of gestation and begins to decline after 15 days of gestation [1]. During postnatal hematopoietic involution, hepatocyte volume rapidly increases, and four types of specialized junctions; i.e., adherens junctions, desmosomes, tight junctions and gap junctions, appear to be fully developed in liver cells [2]. Neonatal livers exami...
متن کاملHigh incidence of spontaneous and chemically induced liver tumors in mice deficient for connexin32
Connexins are subunits of gap junction channels, which mediate the direct transfer of ions, second messenger molecules and other metabolites between contacting cells. Gap junctions are thought to be involved in tissue homeostasis, embryonic development and the control of cell proliferation [1,2]. It has also been suggested that the loss of intercellular communication via gap junctions may contr...
متن کاملPreparation of a gap junction fraction from uteri of pregnant rats: the 28-kD polypeptides of uterus, liver, and heart gap junctions are homologous
A procedure for the preparation of a gap junction fraction from the uteri of pregnant rats is described. The uterine gap junctions, when examined by electron microscopy of thin sections and in negatively stained preparations, were similar to gap junctions isolated from heart and liver. Major proteins of similar apparent molecular weight (Mr 28,000) were found in gap junction fractions isolated ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- The Journal of Cell Biology
دوره 90 شماره
صفحات -
تاریخ انتشار 1981